Restriction enzyme A restriction enzyme or restriction endonuclease is an enzyme that cleaves DNA into fragments at or near specific recognition sites within the molecule known as restriction sites. These are also called Molecular scissors that cut double stranded DNA molecules at specific points. The term restriction enzyme originated from the studies of phage λ, a virus that infects bacteria, and the phenomenon of host-controlled restriction and modification of such bacterial phage or bacteriophage. The phenomenon was first identified in work done in the laboratories of Salvador Luria, Weigle and Giuseppe Bertani in the early 1950s. It was found that, for a bacteriophage λ that can grow well in one strain of Escherichia coli, for example E. coli C , when grown in another strain, for example E. coli K , its yields can drop significantly, by as much as 3-5 orders of magnitude. The host cell, in this example E. coli K , is known as the restricting host and appears to h