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MOLECULAR MECHANISM OF CHROMOSOMAL PAIRING


MOLECULAR MECHANISM OF CHROMOSOMAL PAIRING

Homologous chromosomes start pairing at one or more points along the lengths of chromosomes during this stage. Pairing extends along the rest of the chromosome length in a zipper like fashion. Although heredity, temperature, nutrition, etc.are known to affect pairing, the mechanism of pairing is not well understood. Pairing or synapsis is a very precise process in which only homologous parts o chromosomes come together. Non-homologous parts are looped out and thus remain unpaired. 

A pair of homologous chromosomes is called bivalent .At any one point only two chromosomes can get paired. This is evident when three or four homologous chromosomes are held together to form multivalent, but even then at any given point only two chromosomes are paired. Under the electron microscope the synaptonemal complex appears multilayered. There is a central axis which is flanked on both the sides by an axial element. 

There is a central space in between the central and axial elements. Chromatin is dispersed towards the outer faces of the axial element. DNase destroys the chromatin but not the axial or central elements. These are destroyed by trypsin, indicating thereby that both the central and axial elements are proteinaceous in nature. The central axis is composed of a substance which is synthesized during pairing. Synaptonemal complexes not formed if the homologous are experimentally prevented from pairing. The ends of chromosomes are attached to the inner surface of the nuclear membrane at this stage. A part of the DNA (about 0.3%in lilium) that remains unreplicated during interphase is replicated during this stage of meiosis.




















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